Interaction between P fertilizers and microbial inoculants at the vegetative and flowering stage of Medicago truncatula

Sustainable phosphorus (P) use to meet the global food demand is a major challenge for agriculture. A number of soil microorganisms may influence plants’ ability utilize different chemical forms in rhizosphere. Inoculating phosphate-solubilizing together with rock phosphates (RP) has intrigued researchers over century. However, microbe-mediated mobilization mechanisms RP remain seldomly comprehensively addressed. This study aimed at elucidating these driven by mono- versus mixed-inoculation bacterium (PSB) Pseudomonas corrugata (strain SP77) and arbuscular mycorrhizal fungus (AMF) Rhizophagus irregularis EEZ58) vegetative (Veg) flowering (Flo) stage Medicago truncatula cv. Jemalong. Multivariate analysis combined physiological symbiotic parameters, nutrients concentrations, transcriptional changes P metabolism related genes, showed distinct patterns phenological stages. At Veg stage, P-related were inoculation-type dependent (mono- mixed-inoculation), whereas, Flo they exclusively AMF inoculation-dependent. Competition resources under seems important stage. positive interactions between microbial inoculants appear Variability differentially expressed genes transporters, carotenoid dioxygenase strigolactones abscisic acid (ABA) concentration allowed us elucidate crosstalk among organisms Understanding plants microbes allows development an effective management